Coding

Part:BBa_K1619001:Design

Designed by: Marc-Sven Roell, Caroline Skar Mjønes, and Hanna Noordzij   Group: iGEM15_UiOslo_Norway   (2015-09-01)

medh2 (NAD+ dependent methanol dehydrogenase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

medh2 was amplified from Bacillus methanolicus genomic DNA.

Coding sequence of medh2 was cloned into pSC1B3.

Source

The gene is directly cloned from genomic DNA. Used sequence source for designing primers: http://www.genome.jp/dbget-bin/www_bget?bmet:BMMGA3_09375

References

Müller, J. E. N. et al. Engineering Escherichia coli for methanol conversion. Metab. Eng. 28, 190–201 (2015)